Producing Seedless Strain of Michal Mandarin by Using Gamma Ray

Document Type : Original Article

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Abstract

This research was conducted to obtain seedless fruits of Michal mandarin, taking into account of its distinctive characteristics.  Molecular genetic evaluation among Michal mandarin cultivar and its irradiated cultivar was undertaken by using SCoT and ISSR molecular analysis for PCR reactions. The tested primers revealed a sum of 64 bands. These bands were identified as 53 monomorphic and 11 polymorphic ones with polymorphic (17.18 %) and the polymorphic bands were scored as 11 specific markers. The results showed occurrence of genetic variation according to irradiation treatment that produced eight of positive selected markers were absent in Michal mandarin cultivar and these positive markers may be linked to a new trait and thus we can consider that the irradiated strain from Michal mandarin is a new strain. Moreover, the results demonstrated a considerable resemblance in the majority of fruit attributes between the original cultivar and the new genetic makeup, except for the presence of seeds and a few other characteristics like fruit weight, fruit volume, peel thickness, ascorbic acid.it is suggested that, this new irradiated plant material can be considered as an introduction to the new seedless Michal mandarin strain. Hence, seedless fruits could be produced by irradiating them with 20 Gy of gamma rays.   

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